Use our free Dihybrid Cross Calculator to predict genotypic combinations and phenotypic probabilities of offspring from parents differing in two traits. Based on Mendelian genetics, this calculator simplifies 16-square Punnett analysis into a visual output. Predict offspring traits with this Punnett square calculator.

A dihybrid cross is a genetic cross that studies the inheritance of two different traits at the same time. Each parent contributes two alleles for each trait, and the traits assort independently according to Mendel’s Law of Independent Assortment.

Example: Crossing pea plants with yellow/round seeds (YyRr)

Results in a 4×4 Punnett square showing all combinations.

Classic Phenotypic Ratio:

9 Yellow-Round

3 Yellow-Wrinkled

3 Green-Round

1 Green-Wrinkled

👉 This 9:3:3:1 ratio is typical for a dihybrid cross between two heterozygous individuals.

1. Enter both parent genotypes using four letters (e.g., AaBb, AABB, etc.).
2. Click “Calculate” to generate a full 16-square Punnett output.
3. Review genotype frequencies and their probabilities.

Want a simpler tool? Try our Monohybrid Cross Calculator for single-trait predictions.

Dihybrid crosses usually result in the following phenotypic ratio (when both traits are heterozygous):

• 9 – Dominant for both traits
• 3 – Dominant for first, recessive for second
• 3 – Recessive for first, dominant for second
• 1 – Recessive for both traits

This tool helps students and teachers analyze genetic crosses involving two traits. It’s widely used in biology classes, homework, and genetics labs to understand independent assortment and Mendel’s second law.

How is a dihybrid cross different from a monohybrid?

A monohybrid cross involves only one gene (e.g., Tt x Tt), while a dihybrid cross involves two (e.g., AaBb x AaBb).

How many genotype combinations are possible?

There are 16 combinations in a dihybrid Punnett Square (4×4).

Can I enter homozygous genes?

Yes. Enter any valid 4-letter genotype such as AABB, AaBB, aaBb, etc.

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